mouse anti-tuj 1 antibody  (Covance)

Journal: eNeuro

Article Title: The Auditory Brainstem Response Diagnoses Alzheimer-Like Disease in the 5xFAD Mouse Model

doi: 10.1523/ENEURO.0049-25.2025

Figure Lengend Snippet: No differences in peripheral neuronal structures were observed between genotypes. A , Representative view of SGN labeling in 5xFAD and WT mice at 12M. Images show the 16 kHz regions, with labels for DAPI (blue), Calb2 (green), and TUJ1 (red). B , Quantification of Type 1 SGN density (left) and the proportion of Type 1c SGNs (right). C , Representative view of afferent and efferent terminal labeling in cochlear whole mounts from 5xFAD and WT mice at 12M, with labels for CtBP2 (green) and VAT (magenta). Tonotopic cochlear regions for 8 kHz (left column) and 16 kHz (right column) are shown. D , Quantification of CtBP2 puncta number per IHC (left) and mean VAT terminal volume per IHC (right). E , Representative view of efferent terminal labeling (VAT in white color) in cochlear whole mounts from 5xFAD and WT mice at 12M. The 8 (top row), 16 (middle row), and 32 (bottom row) kHz regions are shown. F , Quantification of VAT terminal volume (µm 3 ) per OHC. Asterisks denote the significance of differences between WT and 5xFAD mice: no significance (ns), p ≥ 0.05; * p < 0.05; ** p < 0.01; and *** p < 0.001, Student's t test or Wilcoxon test. Scale bar, 10 μm for A , C , and E .

Article Snippet: The following primary antibodies were used in this analysis: mouse TUJ1 (anti-beta Tubulin 3, 1:100, R&D Systems) and mouse anti- calbindin 2 (Calb2, 1:100, Millipore Sigma).

Techniques: Labeling

Journal: Scientific reports

Article Title: Metabolic remodeling and calcium handling abnormality in induced pluripotent stem cell-derived cardiomyocytes in dilated phase of hypertrophic cardiomyopathy with MYBPC3 frameshift mutation.

doi: 10.1038/s41598-024-62530-0

Figure Lengend Snippet: Figure 2. Pluripotency of the D-HCM iPSCs and cardiac differentiation of iPSCs into iPSC-CMs. (A). D-HCM iPSCs form several colonies (scale,100 µm). (B). DNA sequencing analysis of D-HCM iPSCs reveals MYBPC3 mutation c.1377delC (red arrow). (C). Immunofluorescence with three pluripotency markers (Nanog, Oct3/4, and Sox2) (scale:50 µm). (D). Flow cytometry of two pluripotency markers (SSEA-4 and TRA-1–60). (E). Immunofluorescence with three germ layer markers: ectoderm (TUJ1), mesoderm (αSMA), endoderm (AFP) (Scale:100 µm). (F). Cardiac induction was performed using the GiWi protocol (Day0-14), purification with a lactate medium (Day15-20), and maturation with a fatty acid medium (Day22-30). In the lower part, photographs show each stage of cardiac differentiation on Day 0, Day 5, Day 10, and Day 50 (Scale:200 µm).

Article Snippet: The antibodies used were anti-mouse TUJ1 (1:100; R&D Systems), anti-mouse SMA (1:100; R&D Systems), anti-mouse AFP (1:100; R&D Systems), and Alexa Fluor 488 goat anti-mouse IgG (1:2000; Thermo Fisher Scientific).

Techniques: DNA Sequencing, Mutagenesis, Immunofluorescence, Flow Cytometry, Purification